Krytptolebias marmoratus embryos were tested for susceptibility to hygromycin B under various concentrations prior to the late blastula stage of development. The concentrations arresting development to the point of lethality for 95% of K. mamoratus embryos within a 48 and 96 h period were 705 mg/l and 601 mg/l, respectively. Nearly half of all embryos were arrested with an effective concentration of 433 mg/l and 207 mg/l for 48 and 96 h, respectively. Data presented here are useful for potential transgenic K. mamoratus (or their embryonic cells) by use of hygromycin B selection for studying the midblastula transition during early development.
Michael J. Bland; Gretchen K. Bielmyer; and Ring, Brian C.
"Toxic Effects of Hygromycin B on Kryptolebias marmoratus Early Development,"
Georgia Journal of Science, Vol. 68, No. 2, Article 6.
Available at: http://digitalcommons.gaacademy.org/gjs/vol68/iss2/6