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Georgia Journal of Science

Abstract

Krytptolebias marmoratus embryos were tested for susceptibility to hygromycin B under various concentrations prior to the late blastula stage of development. The concentrations arresting development to the point of lethality for 95% of K. mamoratus embryos within a 48 and 96 h period were 705 mg/l and 601 mg/l, respectively. Nearly half of all embryos were arrested with an effective concentration of 433 mg/l and 207 mg/l for 48 and 96 h, respectively. Data presented here are useful for potential transgenic K. mamoratus (or their embryonic cells) by use of hygromycin B selection for studying the midblastula transition during early development.

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