GENETIC CHARACTERIZATION OF MUTANTS OF SACCHAROMYCES CEREVISIAE THAT GROW BROWN IN THE PRESENCE OF COPPER
Certain mutants of Saccharomyces cerevisiae produce a brown pigment when grown in the presence of 1 mM CuSO4. Genetic complementation tests indicate that at least three different genes (BRN1, BRN2, and BRN3) can mutate to the brown on copper phenotype. Results from whole genome sequencing studies suggest that BRN1 corresponds to SAM2 and that BRN2 corresponds to CYS3. The failure of BRN3 mutants to complement a deletion of CYS4 indicates that BRN3 corresponds to CYS4. DNA sequencing of additional mutant alleles and segregation analyses support the identities of the three genes. These three genes in yeast encode enzymes that are involved in synthesis of sulfur amino acids and S-adenosylmethionine. Mutations in the human homologs of these genes are associated with genetic diseases. Therefore, we anticipate that these mutants will allow for detailed functional analyses of these genes in yeast and will serve as models of human genetic diseases.
Moore, Nathaniel J.; Schwartz, Brian J.; Rohly, Michael E.; Resch, Christopher L.; and May, Joshua D.
"GENETIC CHARACTERIZATION OF MUTANTS OF SACCHAROMYCES CEREVISIAE THAT GROW BROWN IN THE PRESENCE OF COPPER,"
Georgia Journal of Science: Vol. 75
, Article 48.
Available at: http://digitalcommons.gaacademy.org/gjs/vol75/iss1/48
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