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Abstract

Waxy locus introns from 35 accessions of the species comprising the Cap­sicum annuum complex [C. annuum (7), C. frutescens (10), and C. chinense (11)1, C. baccatum (5), C. chacoense (1) and C. pubescens (1) were cloned and sequenced. These data were combined with existing GenBank waxy intron data on these same species, C. tovarii, C. ciliatum, Lycianthes heteroclita, L. lenta, and L. glandulosa in order to examine the phyloge­netic relationships within this group of plant materials, and to re-examine the strength of the delimitation of the members of the C. annuum complex provided previously by analysis of indel and transition/transversion poly­morphisms at this locus. Included in the subject materials analyzed were genotypes of C. annuum, C. chinense, and C. frutescens that possessed characteristics that were atypical for these respective species. PAUP analy­sis revealed strong support of Capsicum at the genus level. Capsicum ciliatum was the sister species to C. tovarii which was the sister species to a clade containing the five cultivated taxa and C. chacoense. The separation of C. pubescens from other cultivated Capsicum spp. was well supported. The separation of C. annuum from C. chinense and C. frutescens was less strongly supported based on bootstrap values. In contrast, no clear separa­tion of Capsicum chinense from Capsicum frutescens was observed.

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