Hematocrit, hemoglobin concentration, and mean cell hemoglobin concentration (MCHC) were determined in the mangrove killifish, Kryptolebias marmoratus, a tropical fish that can undergo emersion for >1 month when they leave the waters of mangrove forests and find refuge in moist, terrestrial habitats. Hematocrit was 27.67% ± 1.22 (mean ± s.e.m.), hemoglobin concentration was 7.41 ± 0.52 g/dL, and MCHC was 26.47 ± 0.93 g/dL. A linear relationship was present between hematocrit and hemoglobin in K. marmoratus (R2 = 0.833; P < 0.001) . A significant correlation between collected blood volume and standard length was present (P = 0.001). Limitations of the assays used to measure hemoglobin and hematocrit in this small fish species are discussed. Hematocrit and hemoglobin concentrations are within ranges determined for other air-breathing fishes and show the feasibility of measuring hematological parameters in this small fish during emersion.

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