RAPID DETECTION OF CELL ACTIVATION
Rapid detection of infections or monitoring the therapeutic response during the treatments is critical to improve the expected outcome. Currently, available infection diagnostic or monitoring methods are expensive, time-consuming and require well-trained personnel. This study presents rapid and reliable Fourier Transform Infrared (FTIR) spectroscopy to elucidate the alteration in molecular interactions due to infections. As a model for the cell activation due to pathogen infection or allergies, T-cell samples with and without anti-CD3 antibody treatment was used. At the incubation time with the antibody is 75 minutes, a statistically significant alteration (p < 0.02, using paired t-test with post-hoc Bonferroni corrections) in infrared absorbance values of spectral positions at 1367 and 1358 cm-1, was observed. This technique could be useful for precise evaluation of various infectious diseases or allergies and their therapeutic modalities.
Ghimire*, Hemendra; Perera, A. G. Unil; Titus, Jitto; Filfili, Chadi; and Hillard, Julia K.
"RAPID DETECTION OF CELL ACTIVATION,"
Georgia Journal of Science, Vol. 76, No. 1, Article 29.
Available at: https://digitalcommons.gaacademy.org/gjs/vol76/iss1/29