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Georgia Journal of Science

Article Title

EFFECTS OF RESVERATROL CONCENTRATIONS ON THE ARYL HYDROCARBON RECEPTOR IN THE PRESENCE OF INDIGO DYE OR BENZO[A]PYRENE IN HUMAN LIVER CELLS

Abstract

The AHR is a transcription factor that is ligand-activated and controls the expression of a diversified set of genes, including CYP1A1; consequently, exposure to AHR ligands, such as B[a]P and indigo, can increase this receptor’s activation. An increase in the activation of AHR can lead to cancer as some of the ligands that activate it, including B[a]P, are also metabolized by CYP1A1 into highly reactive carcinogens. In this study, we examined how different concentrations of resveratrol could modify AHR activity in human liver cells in the presence of B[a]P or indigo. Based on luciferase reporter gene readings, the co-treatment of resveratrol with the B[a]P or indigo decreased the activation of the AHR, suggesting decreased transcription of the CYP1A1 gene. It was determined that as the concentration of resveratrol increased, the transcription of CYP1A1 decreased in both indigo- and B[a]P-treated cells. The findings of this study suggest that including resveratrol in a dietary plan could act as a preventative measure to combat the constant exposure of AHR-activating ligands through our diet, clothing, and air, decreasing the risk of carcinogenesis.

Acknowledgements

YHC Undergraduate Research Initiative

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