Adenovirus is a double-stranded nonenveloped virus that causes multiple illnesses ranging from the common cold to potentially fatal infections in immunocompromised patients. Part of the infectious cycle of adenovirus involves shutting off host cell protein synthesis and using host cell machinery for viral replication. The life cycle of adenovirus is divided into immediate early, early, and late phases; immediate early proteins control transcription and the cell cycle, and late proteins are structural. It has been suggested that early protein E4 11k may affect gene expression during late phase by disrupting mRNA processing bodies (p-bodies). P-body proteins that have been shown to be disrupted are Ddx6, Lsm1, and Ge-1. Another p-body protein, Pat1b, is a scaffolding protein involved in p-body formation, which also acts as a translational regulator. Pat1b has not been observed in an Ad5 infection. Therefore, our study aims to visualize Pat1b and other cellular proteins via immunofluorescence, to identify their localization and prevalence. Our preliminary results show Pat1b co-localizing with Ddx6 in aggresomes. We also demonstrate that there are more Pat1b foci during an Ad5 infection than in uninfected cells. Alternatively, it has been shown that the number of Ddx6 foci decreases during an Ad5 infection. Currently, we are determining whether this increase in Pat1b foci is due to overexpression of another protein within the cell or simply redistribution of Pat1b, and we also aim to determine whether this pattern of localization is demonstrated in infections of other serotypes.


Georgia College Department of Biological and Environmental Sciences

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