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ANALYSIS OF BIOLOGICAL ACTIVITY OF A NOVEL BIOACTIVE GLASS**

Abstract

Cerium oxide nanoparticles have applications in the field of biomedicine due to their anti-oxidant properties. Nanoceria can function as mimetics of catalase or superoxide dismutase, important antioxidant enzymes found in cells. This property of nanoceria depends upon the Ce+3/Ce+4 ratios found on the surface of nanoceria. In this study, a novel bioactive glass containing nanoceria has been synthesized and biologically characterized for release of nanoceria from the glass as well as the effect of the bioactive glass on cell viability. Several glass samples were synthesized doped with varying concentrations of Cerium (IV) oxide to obtain glass samples embedded with mixed valence nanoceria. The ratio of Ce+3/Ce+4 in the bioactive glass was determined by X-ray Absorption Near Edge Structure (XANES) spectroscopy. These glasses were then incubated in water and release of cerium was determined by Inductively coupled plasma - optical emission spectrometry. Initial experiments show that 18% of cerium present in the bioactive glass is released in the first two hours of incubation in water. Glass sample that contains no nanoceria completely dissolves in water. The MTS cell proliferation assay was used to determine growth and viability of cells in presence of bioactive glass containing nanoceria with different Ce3+/Ce4+ ratios. Preliminary cell viability assays indicate that glass samples containing nanoceria stimulate cell growth of hamster kidney epithelial cells (BHK cells) when compared to the cells treated with glass containing no cerium. This study provides evidence for successful synthesis of a novel bioactive glass that functions as a creator and carrier of mixed valence nanoceria. The novel bioactive glass is able to dissolve and release nanoceria that can be tested on several cell-lines and has the potential to be a potent therapeutic agent for pathologies caused by oxidative stress.

Acknowledgements

UNG Dept. of Biology

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