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Georgia Journal of Science

Article Title

DETERMINING WHETHER TURF BEAD EXTRACT IS MUTAGENIC IN THE PRESENCE OF CYP1A1**

Abstract

A recent trend in sports field management is to utilize artificial turf that are seeded with rubber beads. There is a growing concern that toxic chemicals might be leaching from these turf beads, placing the athletes at an increased risk of cancer. Previous studies in our lab have shown that chemicals extracted from these beads, using either water or DMSO as solvents, can upregulate CYP1A1 activity. Transcription of CYP1A1 is mediated by the aryl hydrocarbon receptor (AHR). Benzo[a]pyrene (BAP) is a known ligand of the AHR, induces transcription of CYP1A1, and is then metabolized by CYP1A1 into its reactive diol epoxide form. This metabolite can cause mutations and DNA damage. In these studies we will examine the ability of bead extracts to cause DNA mutations using a modified Ames test (supplemented with CYP1A1 enzymes). Further, methylphenidate (Ritalin), a known inhibitor of CYP1A1 activity, will be combined with the BAP treatment in an attempt to limit DNA damage through this pathway.

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