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A BLIND STUDY ON THE PREVALENCE OF ANABOLIC STEROID CLENBUTEROL AMONG COLLEGIATE ATHLETES AND THE EFFECTIVENESS OF GOLD NANOPARTICLE LATERAL FLOW IMMUNOASSAY TEST-KITS.

Abstract

Clenbuterol is a beta2 agonist with implications in food poisoning and anabolic-adrenergic lean muscle growth effects. Symptoms of clenbuterol intoxication includes, but are not limited to tachycardia, sweating, and anxiety, but despite negative symptomatology, clenbuterol is used as a lean muscle drug stimulant in athletes, non-athletes, and in raising livestock animals. Because of the potential dangerous misuse of clenbuterol, there is a market for detection methods to detect clenbuterol in various mediums. Gold nanoparticle immunoassays are effective tools that make use of the biological compatibility in sensory actions of gold nanoparticles to qualitatively detect clenbuterol. With a gold nanoparticle immunoassay it is possible to quickly and effectively test athletes on a college campus for the presence of clenbuterol, whom either use clenbuterol for athletic purposes or came into contact with tainted meat. With the usage of “Creative Diagnostics” clenbuterol test strips, a blind study on the prevalence of clenbuterol amongst Brewton-Parker College men's and women’s soccer, track, wrestling teams, as well as a random group, was conducted. The study found a campus sample prevalence of 4% among all test participants, and a 2% prevalence among athletic groups. The study did not find a statistically significant difference when comparing male and female prevalence.

Acknowledgements

Division of Math & Natural Sciences, BPC

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