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Georgia Journal of Science

Article Title

Establishment of CRISPR/Cas9-Aided Knockout of the ZIC2 gene in the African American Prostate Cancer Cell Line E006AA-Pr.

Abstract

The largest U.S. cancer health disparity exists in prostate cancer (PCa), with African American (AA) men having the highest incidence and mortality rates. Prostate cancer is the second leading cause of cancer-related death in African American men. The present study evaluated the effects of ZIC2 and the possible underlying mechanisms in the E006 parental African American cell line that is shown to be highly tumorigenic and produced tumors at an accelerated growth rate because of the increase of ZIC2 genes in African American males. The zinc finger transcription factor ZIC2 has been reported to be highly expressed in human cancers. This study analyzed the experimental research that the overexpression of ZIC2 contributes to the progression of prostate cancer. E006AA cells with either overexpressed ZIC2 or suppressed ZIC2 were analyzed to determine cell viability, colony formation, cell proliferation and immunoblot assays. The expression levels of ZIC2 were analyzed by CRISPR-Cas9, Western blot, proliferation growth curves and PCR analysis and we discovered using these experimental techniques to knockout the ZIC2, reduced cell proliferation occurred. This research investigated the role of ZIC2 in prostate cancer progression and the effects of the loss or gain of function of ZIC2 by using CRISPR-Cas 9 genome editing technology.

Acknowledgements

Young Harris Undergraduate Research Initiative

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