AN ECONOMICAL SYNTHETIC ROUTE IN THE OCEAN FOR THE MARINE NATURAL PRODUCT, BRYOSTATIN

Authors

Thomas Falkenhausen*, Valdosta State University, Valdosta, GA 31698Follow
Torien Beard*, Valdosta State University, Valdosta, GA 31698
Tom Manning Dr., Valdosta State University, Valdosta, GA 31698Follow

Abstract

Bryostatin is a marine natural product found in minute amounts in nature. Cost 17 million USD/gram, this molecule targets latent HIV reservoirs, has potential for slowing the progression of Alzheimer's, and demonstrates strong anti-cancer properties. We have developed an economical synthesis of bryostatin through the creation of a bioreactor that cultivates a symbiotic bacterium which lives on the bryosome B. Nerintina. This bioreactor is comprised of inexpensive organic materials providing essential nutrients which promotes the growth of the bacterium. Our bioreactor was deployed off the Gulf Coast for a short period to attract our symbiotic bacterium. This bioreactor was returned to the lab where it was monitored weekly for 2 months with sample extraction every other day. Each sample was labeled and stored in cold storage to prevent the degradation of bryostatin. After our collection period, we ran each sample through a UV Spectrometer to determine the possible presence of Bryostatin. Promising samples were, then, ran through a liquid chromatography mass spectrometer to determine the different types of Bryostatin present.

Acknowledgements

NSF, NCI

Recommended Citation

Falkenhausen*, Thomas; Beard*, Torien; and Manning, Tom Dr. (2020) "AN ECONOMICAL SYNTHETIC ROUTE IN THE OCEAN FOR THE MARINE NATURAL PRODUCT, BRYOSTATIN," Georgia Journal of Science, Vol. 78, No. 1, Article 29.
Available at: https://digitalcommons.gaacademy.org/gjs/vol78/iss1/29