BLOODROOT EXTRACT DECREASES TNF-α LEVELS IN THP-1 CELLS
Studies have shown that sanguinarine, which is isolated from the bloodroot plant (Sanguinaria canadensis), has anti-inflammatory properties. TNF-α is cytokine that is created by cells, including macrophages, in response to an inflammatory insult. THP-1 monocytes, which can differentiate into macrophages following phorbol 12-myristate 13-acetate (PMA) exposure, are a common model used for examining inflammation. Lipopolysaccharide (LPS) treatment of THP-1 cells has been shown to cause an increase in TNF-α expression. In our studies, we tested THP-1 cells exposed to LPS in the presence or absence of bloodroot extract to determine if pre-treatment of the cells with these extracts can prevent TNF-α expression. Extracts were made using 0.125 g/ml of dried bloodroot and either DMSO, ethanol, or hot water as the solvent. TNF-α levels were determined using an ELISA kit and compared to provided standards. Results indicate that all three of our bloodroot extracts were able to decrease TNF-α expression by more than 99.9% (p<0.01). These results indicate that bloodroot extract, dissolved in any of the three solvents tested, prevents TNF-α from being produced in differentiated THP-1 cells. Our findings suggest that bloodroot extracts may be useful in the prevention or treatment of inflammation.
YHC Undergraduate Research Initiative
Girle, Stephanie D. and Schroeder, Jennifer C.
"BLOODROOT EXTRACT DECREASES TNF-α LEVELS IN THP-1 CELLS,"
Georgia Journal of Science, Vol. 78, No. 1, Article 76.
Available at: https://digitalcommons.gaacademy.org/gjs/vol78/iss1/76