THE EFFECT OF ADENOVIRUS E4ORF3 AND DDX6 ON RIG-I MEDIATED INTERFERON RESPONSE**
The cytosolic pattern recognition receptor, retinoic-acid inducible gene I (RIG-I), plays a key signaling role in initiating the type-1 interferon (IFN1) host response in the presence of a virus. RIG-I senses viral RNA in the host cell and activates a signaling cascade that leads to the production of IFN1. The function of RIG-I has been frequently studied during an infection with an RNA virus, however studies of RIG-I with DNA viruses are not as abundant. Adenovirus is a nonenveloped, double-stranded DNA virus containing early proteins that function to create a more advantageous host-cell environment for viral replication. Adenovirus early region 4 11 kilodalton protein (E4 11k) is known to relocalize host-cell target proteins with functions ranging from double-strand break repair to mRNA regulation. One structure that is targeted for relocalization is the P body, which is involved in cytoplasmic RNA regulation and mRNA degradation. One major P body protein that E4 11k interacts with is DEAD-Box Helicase 6 (Ddx6), that acts as an RNA helicase and has functions in mRNA decapping. More recently, Ddx6 has been found to bind to RIG-I and is known to have both antiviral and pro-viral roles during infection. It is important to note that adenovirus infection has been shown to induce IFN-ß in a biphasic manner, with the late phase being RIG-I dependent. This project aims to look at the possible inhibitory effects of the E4 11k/Ddx6 interaction on RIG-I during an adenovirus infection. Cells will be infected with wild type or E4 11k-deletion mutants of adenovirus and then harvested for qRT-PCR at various hours post infection using primers for RIG-I, IFN-ß, GAPDH, and the virus associated RNA (VA1). Our results will help to create a better understanding of how the host innate immune response is activated and possibly subverted in DNA viruses.
GCSU Dept. of Environmental and Biological Sciences
Ginn, Jadalynn A. and Karen, Dr. Kasey A.
"THE EFFECT OF ADENOVIRUS E4ORF3 AND DDX6 ON RIG-I MEDIATED INTERFERON RESPONSE**,"
Georgia Journal of Science, Vol. 80, No. 1, Article 52.
Available at: https://digitalcommons.gaacademy.org/gjs/vol80/iss1/52