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Determining minimum inhibitory concentration of sodium salicylate and developing PCR-based identification of Providencia rettgeri and Providencia stuartii

Abstract

The genus Providencia comprises of urease-producing bacteria accountable for an extending range of human infections. Urinary tract infections (UTIs) are a common disease related to Providencia that’s present in patients with long-term urinary catheters. Salicylate and related compounds, commonly present in aspirin, have a variety of effects in eukaryotic systems and are well known for their medicinal properties. Salicylate also has numerous effects on bacteria, yet only a handful of individuals within the scientific community appreciate these findings. This study investigates the effects of sodium salicylate on the growth of P. rettgeri and P. stuartii. Minimum inhibitory concentrations (MICs) of sodium salicylate were determined by using broth dilution methods. Both P. rettgeri and P. stuartii were grown overnight in tryptic soy broth (TSB) with different concentrations of sodium salicylate to determine MICs of sodium salicylate. MICs of sodium salicylate on the growth of P. rettgeri was determined at 17mM – 20mM and MICs for P. stuartii was determined at 30mM – 35mM. Based on cultural and morphological identification, pathogen detection and identification methods are time consuming and not always reliable. A polymerase chain reaction (PCR)-based pathogen detection method with the genus-specific primers is fast and reliable for detecting Providencia species. We have deigned two pairs of primers based on the global regulatory gene rsmC to detect P. rettgeri and P. stuartii. This study will be helpful to control the growth and break down antibiotic resistance by using sodium salicylate on the growth of Providencia species. Also, PCR based tools of this study should make easier the detection of P. rettgeri and P. stuartii.

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