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EXTRACTION OF CAROTENOIDS USING DEEP EUTECTIC SOLVENTS AS GREEN AND EFFICIENT SOLVENTS**

Abstract

The extraction of bioactive compounds from natural products using conventional organic solvents can leave large traces of organic solvents behind. This can lead to pollution in the environment and can also leave traces of the organic solvents in the extracts. On top of having such a negative impact on the environment the extraction yields obtained are fairly low. It is thus important to find an alternative method of extraction that can solve these problems. Deep eutectic solvents (DESs) can be used as an alternative as they are considered more environmentally friendly, have low toxicity, low cost, and can give better extraction yields. In this study, a wide range of DESs will be synthesized for the extraction of lycopene and betacarotene from tomato paste and paprika, respectively. The experimental approaches include sonication bath, vortexing, or centrifugation to obtain a proper mixture from which the carotenoids are being extracted. The carotenoid extracts are analyzed using a UV spectrophotometer and HPLC equipment. It is expected that using a DES solvent instead of a conventional organic solvent will reduce toxicity and will also increase extraction yield because of the nonpolar properties of DES solvents. Sufficient data from this study can induce a change that can make extractions environmentally friendly.

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