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EFFECTS OF CANNABIDIOL AND OMEPRAZOLE ON CELL GROWTH AND INTRACELLULAR LACTATE LEVELS IN MOUSE LIVER CELLS**

Abstract

Cannabidiol (CBD) is a non-psychoactive component of cannabis oil that is approved for certain medical uses. Numerous clinical trials are currently exploring its effectiveness in managing symptoms associated with diabetes mellitus. CBD is a ligand of the aryl hydrocarbon receptor (AhR), which has been shown to regulate intracellular lactate levels. Diabetics, especially when in ketoacidosis, are known to have increased lactate levels. Omeprazole, a common treatment for acid reflux available in both prescription and over-the-counter formulations, is also an AhR ligand. In these studies, we explore the effects of CBD (0.03-20 μM) and omeprazole (25-100 μM) on levels of lactate in the mouse hepatocyte cell line, Hepa 1.1, using a colorimetric L-lactate assay. We also examine morphological changes to the cells and overall protein content in the samples following treatment to detect changes in cell growth. Initial observations indicate that higher concentrations of either CBD or omeprazole decrease cell growth, resulting in lower lactate levels in those samples, as compared to the negative control samples treated with only ethanol and DMSO. A Bradford Assay will be used to normalize lactate levels to total protein in the samples.

Acknowledgements

YHC Undergraduate Research for the Common Good

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