FLUORESCENT AND COMPARATIVE ANALYSIS OF PROTEIN LOCALIZATION IN SACCHAROMYCES CEREVISIAE**

Authors

Lewis S. Barr*, Georgia College and State UniversityFollow
Ellen France, Georgia College and State UniversityFollow

Abstract

A principal objective of cellular biology is to identify the function of proteins as they interact with their environment. Cellular localization provides context for the function and interactions of proteins. In this study, we seek to identify the localization of three proteins within Saccharomyces cerevisiae through C-terminal green fluorescent protein epitope tagging and subsequent fluorescent microscopy. As 75% of the yeast proteome has been classified, this project seeks to fill the remaining gap in unidentified cellular localization by investigating the proteins fsh3, gid10, and ade13. The analysis of high-resolution localization and comparative phylogenetic data reveals potential cellular environments and protein functions.

Acknowledgements

Sophia McNeill, Dr. Richard Adams, Dr. Indiren Pillay, and Dr. Ashok Hegde

Recommended Citation

Barr*, Lewis S. and France, Ellen (2023) "FLUORESCENT AND COMPARATIVE ANALYSIS OF PROTEIN LOCALIZATION IN SACCHAROMYCES CEREVISIAE**," Georgia Journal of Science, Vol. 81, No. 1, Article 4.
Available at: https://digitalcommons.gaacademy.org/gjs/vol81/iss1/4