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ISOLATION, CHARACTERIZATION, AND BIOACTIVITY OF SECONDARY METABOLITES IN EUPATORIUM SEROTINUM **

Abstract

Phytochemistry, a subset of natural products chemistry, pertains to the study of chemically derived products from natural substances. Findings from this field have important applications in medicinal research due to the distinctive properties of plant metabolites. All metabolites are produced from the metabolic cycle. Primary metabolites contribute to organism growth, development, and maintenance of energy. Secondary metabolites, on the other hand, are more unique with specialized functions. The naturally defensive and healing properties of natural products correlate to the organisms' secondary metabolites. These compounds are found in small quantities compared to primary metabolites and can vary greatly in structure, function, and formula depending on the plant. Harnessing and studying the properties of secondary metabolites may contribute to advanced medicine through sustainable biopharmaceutical practices. Eupatorium Serotinum serves as the specimin of interest for its fever reducing qualities. Isolation and seperation of metabolites is performed through Dry Column Vacuum Chromatography (DCVC) using solvents of various polarity. Analysis of the seperated components is then performed through High Performance Liquid Chromatography (HPLC), Gas Chromatography/Mass Spectrometry (GC/MS), and Thin Layer Chromatography (TLC). The seperated fractions are also tested for growth inhibition against E. coli bacteria strains to reveal potential antibiotic properties.

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