TECHNIQUES FOR ANALYZING ATR-FTIR SPECTRA APPLIED TO MOUSE MODEL SERA**

Authors

Michael R. Nelson, Georgia State University,Atlanta, GA 30303Follow
Mehmood Pirzada, Georgia State University,Atlanta, GA 30303Follow
Amous Toure, Georgia State University,Atlanta, GA 30303Follow
Mathes Dayananda, Georgia State University,Atlanta, GA 30303Follow

Abstract

Fourier Transform Infrared Spectroscopy offers a window into biological systems at a resolution of individual molecular vibrations. These vibrations often present themselves within an ensemble of other Gaussian oscillators. This necessitates different techniques in spectral analysis to determine the nature of the underlying bands present in FTIR spectra. This article presents some of these techniques with examples derived from previously studied ATR-FTIR spectra of mouse-model colitis sera. Differences in biomolecular content between samples are analyzed and compared using Gaussian curve-fitting, spectral integration, the Beer-Lambert Law, and normalized spectrum subtraction. The physical interpretations and limitations of each technique are also discussed.

Recommended Citation

Nelson, Michael R.; Pirzada, Mehmood; Toure, Amous; and Dayananda, Mathes (2024) "TECHNIQUES FOR ANALYZING ATR-FTIR SPECTRA APPLIED TO MOUSE MODEL SERA**," Georgia Journal of Science, Vol. 82, No. 1, Article 89.
Available at: https://digitalcommons.gaacademy.org/gjs/vol82/iss1/89