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IDENTIFYING COLITIS OF TWO MICE MODELS USING ANALYSIS OF FTIR SPECTROSCOPY

Abstract

Inflammatory bowel disease (IBD) involves chronic and progressive inflammation of the gastrointestinal tract, characterized by complex metabolic and immunological changes. Mouse models can play a critical role in understanding these processes. Dextran sodium sulfate (DSS)-treated mice develop chemically-induced colitis, while interleukin-10 knockout (IL-10) engineered mice are bred to naturally develop chronic colitis. Together, these models represent two distinct developments of IBD and provide an opportunity to compare biomolecular differences. Fourier-transform infrared spectroscopy (FTIR) is an analytical technique that shines light on a sample to detect how different molecular vibrational bonds absorb energy. This absorption creates a unique “fingerprint” that reflects the chemical makeup of the sample. FTIR is fast, requires minimal sample preparation, and can detect changes in bio-molecules associated with IBD, including changes in protein, lipid, and carbohydrate regions. In this research, statistical analysis is applied to FTIR spectra of mouse serum samples. My end goal is to determine the underlying spectral biomarker changes within spectral regions that can discriminate between the DSS and IL-10 models of colitis. Here, I will discuss the current findings related to this focus and emphasize their significance to the study.

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