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IDENTIFYING SPECTRAL BIOMARKERS OF IBD USING ANALYSIS OF FTIR SPECTROSCOPY

Abstract

Inflammatory Bowel Disease (IBD) is defined as a group of diseases marked by chronic and significant inflammation of the gastrointestinal system, which involves complex immunological and biochemical factors. IBD requires early detection to reduce disease burden. However, current diagnostic techniques lack accuracy. Endoscopy and colonoscopy, while the diagnostic gold standard, are invasive and costly procedures with possible risks and poor patient acceptance. This makes them particularly unsuitable for elderly and pediatric patients. The two forms of mice results reported here are from standard models for evaluating the biomarker alterations due to IBD. Interleukin-10 knockout (IL-10) mice are mice that have been genetically altered to develop colitis. The dextran sodium sulfate (DSS)- treated mice are administered DSS to induce colitis. Our research study uses Fourier-Transform Infrared Spectroscopy (FTIR) to analyze DSS and IL-10 mouse model samples using infrared light absorption in samples. FTIR offers a more rapid approach for detecting vibrational modes in the serum samples. Specific spectral regions of samples can show distinct spectral biochemical signatures, noted as Spectral Biomarkers (SBMs). The research I will present will be on current results towards identifying more statistically significant discriminatory SBMs of colitis for the two mouse samples. Such SBMs can support faster screening approaches for IBD and can contribute to ongoing efforts to use spectroscopy as a more patient friendly, cost-effective, minimally invasive diagnostic tool.

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